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anti galectin 3 mac2 primary antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti galectin 3 mac2 primary antibody
    Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with <t>Mac2</t> (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Anti Galectin 3 Mac2 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+galectin+3+mac2+primary+antibody/galectin-3+Antibody/pmc09582277-67-21-26
    Average 95 stars, based on 385 article reviews
    anti galectin 3 mac2 primary antibody - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis"

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2022.881848

    Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with Mac2 (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with Mac2 (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Control, Staining, Immunofluorescence, Fluorescence, Immunohistochemical staining, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

    Related Articles

    Incubation:

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis
    Article Snippet: The liver tissue was blocked with BLOXALL blocking solution (Vector Laboratories, #SP-6000) for 10 min, washed three times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4°C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis.
    Article Snippet: *p < 0.05, **p < 0.01, ***p < 0.001. times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4◦C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.

    Avidin-Biotin Assay:

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis
    Article Snippet: The liver tissue was blocked with BLOXALL blocking solution (Vector Laboratories, #SP-6000) for 10 min, washed three times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4°C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis.
    Article Snippet: *p < 0.05, **p < 0.01, ***p < 0.001. times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4◦C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis
    Article Snippet: The liver tissue was blocked with BLOXALL blocking solution (Vector Laboratories, #SP-6000) for 10 min, washed three times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4°C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis.
    Article Snippet: *p < 0.05, **p < 0.01, ***p < 0.001. times with 1X PBS, and blocked using 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) for 1 h at room temperature. .. The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4◦C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum. .. The liver tissue was washed three times with 1X PBS and incubated with biotinylated Secondary antibody (Invitrogen, #B2770) for 1 h at room temperature.



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    Santa Cruz Biotechnology anti galectin 3 mac2 primary antibody
    Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with <t>Mac2</t> (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with <t>Mac2</t> (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with Mac2 (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Medicine

    Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis

    doi: 10.3389/fmed.2022.881848

    Figure Lengend Snippet: Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with Mac2 (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4°C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum.

    Techniques: Control, Staining, Immunofluorescence, Fluorescence, Immunohistochemical staining, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Expressing